PharmD Info

A forum for Indian Pharmacy Professionals

Pharmaceutical instruments which are used for analysis, formulation, drug development etc are discussed. e.g. HPLC, Friability tester
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High-performance liquid chromatography (HPLC) is an essential analytical technique in the pharmaceutical industry, and the development of innovative HPLC column technologies has significantly enhanced its performance and efficiency. In this article, we will explore some of the latest HPLC column technologies and their benefits for improving analytical accuracy and speed.

Monolithic columns: Unlike traditional packed columns that are filled with small beads, monolithic columns consist of a single piece of porous material. This design allows for faster flow rates and higher sample throughput, making them ideal for high-speed separations. Additionally, monolithic columns offer better mechanical stability and lower backpressure compared to traditional columns.

Core-shell columns: These columns feature a solid core with a thin porous shell, providing higher efficiency and faster separations than traditional fully porous columns. The core-shell design allows for higher flow rates and increased resolution, resulting in improved analytical speed and sensitivity. Additionally, these columns are more robust and can withstand higher pressures and flow rates, reducing the risk of column damage and increasing column lifespan.

Fused-core columns: Similar to core-shell columns, fused-core columns feature a solid core with a thin porous shell. However, the core and shell materials are fused together, resulting in a more uniform particle distribution and higher packing density. This design allows for faster and more efficient separations than traditional fully porous columns, with improved peak symmetry and resolution. Fused-core columns also offer excellent mechanical stability and low backpressure, reducing the risk of column damage and improving column lifespan.

Ultra-high-performance liquid chromatography (UHPLC) columns: UHPLC columns feature smaller particle sizes and narrower particle size distributions than traditional HPLC columns, allowing for higher resolution and faster separations. These columns can operate at higher pressures and flow rates, providing faster and more efficient separations with improved peak symmetry and sensitivity. UHPLC columns are also compatible with smaller injection volumes, reducing sample volume requirements and increasing sensitivity.

In conclusion, innovative HPLC column technologies have significantly improved the performance and efficiency of HPLC, offering faster separations, higher resolution, improved sensitivity, and reduced sample volume requirements. Monolithic, core-shell, fused-core, and UHPLC columns are just a few examples of the latest HPLC column technologies that are driving innovation and advancement in the pharmaceutical industry. By adopting these cutting-edge technologies, pharmaceutical companies can enhance their analytical accuracy and speed, resulting in faster and more efficient drug development processes.
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